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27-02-2024
Biology

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Devise an experiment, based on sucrose gradient sedimentation, that would demonstrate that proteins are synthesized on polyribosomes.
a) Centrifuge the cell lysate on a sucrose gradient
b) Perform SDS-PAGE analysis on the sucrose gradient fractions
c) Label newly synthesized proteins with radioactive amino acids
d) Measure the absorbance of each sucrose gradient fraction at 260 nm

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